Composite

Part:BBa_K4790091:Design

Designed by: Yutong Ji   Group: iGEM23_NNU-CHINA   (2023-10-11)


ASR1(node 59)-F213S/pET-22b(+)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 960
    Illegal PstI site found at 261
    Illegal PstI site found at 393
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 960
    Illegal NheI site found at 882
    Illegal NheI site found at 1113
    Illegal PstI site found at 261
    Illegal PstI site found at 393
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 960
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 960
    Illegal PstI site found at 261
    Illegal PstI site found at 393
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 960
    Illegal PstI site found at 261
    Illegal PstI site found at 393
    Illegal NgoMIV site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We should select direction of mutation which is predicted the highest possibility. Mutation should refer to Codon preference in E.coli.


Source

It does not come from any genomic sequence. Node 59 comes from Ancestral Sequence Reconstruction (ASR). The mutation site is predicted by machine learning.

References